Re: plastic immuno

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From:Boneslides@aol.com
To:histonet@pathology.swmed.edu
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In a message dated 03/21/2000 4:55:19 AM Eastern Standard Time, 
uvsgc@msu.oscs.montana.edu writes:

<< Have
 seen his slides, really wonderful staining! and was impressed it could be 
done
 by removing MMA with warm xylene.  
 
 Hoods are a must! >>

I de-plasticize my MMA sections with methyl acetate rather than xylene (warm 
or cold!).  It works very well in 20-30 minutes.  I don't know how it would 
affect immunos.  

Diane Mahovlic
Orthopedic Pathology Laboratory
The Cleveland Clinic Foundation
Cleveland, Ohio



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