Re: help with transfering plastic sections
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From: | Roger Moretz <stamptrain@yahoo.com> |
To: | Roger Moretz <stamptrain@yahoo.com>, Gary Radice <gradice@richmond.edu>, HistoNet Server <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
In reading my initial posting, I realized I had failed
to complete my thought. After picking up the section
on the glass rod/toothpick, it can be rolled out onto
the surface of the water. I have done this both onto
a water bath (usually a glass staining dish) or
directly onto a drop on a slide.
Roger Moretz
--- Roger Moretz <stamptrain@yahoo.com> wrote:
> Gary:
> Don't know if this will work for you, but I used to
> (when I did a lot of methacrylate work in the late
> 70's) pick up the sections from the knife by rolling
> them around a fine glass rod or even a wooden dowel
> (actually a round toothpick). It takes practice and
> a
> lot of patience, but this was the most reliable
> method
> I have found.
>
> Roger Moretz
> Dept of Toxicology
> Boehringer Ingelheim Pharmaceuticals, Inc.
>
> --- Gary Radice <gradice@richmond.edu> wrote:
> > We are cutting glycolmethacrylate sections, around
> 1
> > micrometer thick, with
> > glass knives and are having a difficult time
> > transfering sections to
> > microscope slides without the sections folding.
> How
> > do you do it/ Does
> > anyone have a favorite technique they would care
> to
> > share?
> >
> > Gary P. Radice gradice@richmond.edu
> > Associate Professor of Biology 804 289 8107
> (voice)
> > University of Richmond 804 289 8233 (FAX)
> > Richmond VA 23173
> > http://www.science.richmond.edu/~radice
> >
> >
> >
> >
>
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