Re: help with transfering plastic sections

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From:Roger Moretz <stamptrain@yahoo.com>
To:Gary Radice <gradice@richmond.edu>, HistoNet Server <histonet@pathology.swmed.edu>
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Gary:
Don't know if this will work for you, but I used to
(when I did a lot of methacrylate work in the late
70's) pick up the sections from the knife by rolling
them around a fine glass rod or even a wooden dowel
(actually a round toothpick).  It takes practice and a
lot of patience, but this was the most reliable method
I have found.

Roger Moretz
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.

--- Gary Radice <gradice@richmond.edu> wrote:
> We are cutting glycolmethacrylate sections, around 1
> micrometer thick, with
> glass knives and are having a difficult time
> transfering sections to
> microscope slides without the sections folding. How
> do you do it/ Does
> anyone have a favorite technique they would care to
> share?
> 
> Gary P. Radice			gradice@richmond.edu
> Associate Professor of Biology		804 289 8107 (voice)
> University of Richmond		804 289 8233 (FAX)
> Richmond VA 23173	
> http://www.science.richmond.edu/~radice
> 
> 
> 
> 

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