Re: Survey
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| From: | Katri Tuomala <katri@istar.ca> |
| To: | denise M m Long-Woodward <denisew2@juno.com> |
| Reply-To: | |
| Content-Type: | text/plain; charset=us-ascii |
Hi Denise,
Our IF protocol for renal biopsies:
Cut 5mu frozen sections and pick up on poly-L-lysine coated slides.
Air dry for 30 minutes. (No fixation)
Wash 15 minutes in PBS.
Apply FITC conjugated antibodies.
Wash 2x15 minutes in PBS. Coverslip.
Katri
Katri Tuomala
Anatomic Pathology
St.Joseph's Hospital
Hamilton, Ontario, Canada
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