Re: Signal Amplification
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From: | amos brooks <atbrooks@snet.net> |
To: | Jay Turner <turnerjayd@hotmail.com> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Hi,
DAKO makes a Catalyzed Signal Amplification Kit. This amplifies the signal
enough to cause an antibody that usually doesnt stain on FFPE (formalin fixed
paraffin embedded) tissues. The procedure is a bit complicated and rather
expensive but the results can be quite quite good.
Ventana also makes a one step amplification reagent that I've heard is good
although I've not used it much.
Amos Brooks
Jay Turner wrote:
> I am staining a fractured rat femur with an immunohistochemical stain to
> IGF-1. The stain is a standard biotin-avidin peroxidase with DAB. The
> samples were decalcified in Cal-Ex (Fisher), it was a little harsh. There
> is stain in the section but it is very faint. I don't want to re-do these
> animals and re-decalcify. I have tried to use a metal enhanced DAB (cobalt)
> but the color is too close to the hematoxylin counterstain. Is there any
> other way to enhance DAB and the staining? Any suggestions? Is
> fluorescence the way to go?
>
> Thanks!
>
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