Re: Signal Amplification

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From:amos brooks <>
To:Jay Turner <>
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    DAKO makes a Catalyzed Signal Amplification Kit. This amplifies the signal
enough to cause an antibody that usually doesnt stain on FFPE (formalin fixed
paraffin embedded) tissues. The procedure is a bit complicated and rather
expensive but the results can be quite quite good.
    Ventana also makes a one step amplification reagent that I've heard is good
although I've not used it much.
Amos Brooks

Jay Turner wrote:

> I am staining a fractured rat femur with an immunohistochemical stain to
> IGF-1.  The stain is a standard biotin-avidin peroxidase with DAB.   The
> samples were decalcified in Cal-Ex (Fisher), it was a little harsh.  There
> is stain in the section but it is very faint.  I don't want to re-do these
> animals and re-decalcify.  I have tried to use a metal enhanced DAB (cobalt)
>   but the color is too close to the hematoxylin counterstain.  Is there any
> other way to enhance DAB and the staining?  Any suggestions?  Is
> fluorescence the way to go?
> Thanks!
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