I like the Pinkerton's method of the Machavellos---staining in methylene
blue solution overnight and with basic fuchsin solution for 30
minutes.  In my work (animal tissues) I found it to be the best of the
differential stains for coxiella.  However we now use an IHC for
confirmation.

Pam Dilbeck
Microbiologist
Washington Animal Disease Diagnostic Laboratory


On Tue, 14 Mar 2000, Joan C. Yonchek wrote:

> 
> 
> What stains are folks using to demonstrate
> coxiella burnetti, the organism responsible
> for Q Fever?
> 
> Thanks,
> Joan
> Department of Orthopaedics and Rehabilitation
> University of Florida
> PO Box 100246
> Gainesville, Florida  32611
> Tel: 352 392 7570
> Fax: 352 392 8637
> Email: yonchjc@ortho.ufl.edu
> 
> 

	Pam Dilbeck
	Immunohistochemistry Section
	Washington Animal Disease Diagnostic Laboratory