Re: Method for double immuno staining
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From: | "Thomas J. Kuwahara" <tom@adpath.com> |
To: | cklein@mail.mdanderson.org |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Dear Christine:
Would like to recommend method manual written by a good friend of mine
for DAKO. Called Immunoenzymatic Double Staining Methods, A Practical
Guide. Got my copy from Europe. Don't know if it is available now from
the US DAKO group. Call them at 800-235-5763. I think some info may
also be available on the histonet archive website,
http://www.pathsearch.com/histonet.html
Tom
cklein@mail.mdanderson.org wrote:
>
> A doctor has asked our lab to do a double immuno stain using both p21 and ki 67.
> My question is---where can I look for a relatively easy to understand method to
> accomplish this? I suspect that I will need to use 2 different chromagens(dab
> and aec) but do I have to retreive the antigens twice, block peroxidase twice,
> etc?
> My assumption is that I should proceed with one antibody stain form start to
> finish and then for the second antibody I should start the procedure from the
> point of primary antibody application to the second chromagen.
> Any help anybody can give regarding either the method or where to look up the
> method would be greatly appreciated.
> Sincerely,
> Christine Klein
> MD Anderson Cancer Center
> Houston, Tx.
--
Thomas J. Kuwahara - Senior Immunohistochemist
Resolution Sciences Corporation - http:www.resolve3d.com
3801 Sacramento St., Suite 621, San Francisco, CA 94118
T: 415/750-6800, ext23067 F: 415/750-2332 E: tom@resolve3d.com
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