Tina,

The idea here is to leave the blocking solution on to continue the blocking 
action for the primary antisera. Rinsing the blocking serum off may result 
is less efficient blocking of non-specific sites. Draining will get rid of 
the excess but won't significantly dilute the primary.

Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

Phone: (404) 639-3964
FAX:  (404)639-3043


----Original Message Follows----
From: Montina Van Meter <mvanmete@postbox.acs.ohio-state.edu>
To: histonet@pathology.swmed.edu
Subject: IHC question
Date: Tue, 21 Mar 2000 09:27:49 -0500

Dear Histonetters:
		Can someone provide information as to why most IHC protocols have you
drain off the blocking solution before adding the primary and not rinsing
with buffer in between?  In all the procedures I have looked at they
explain the theory behind the other steps but not this one.  Thanks in
advance.

						Tina


Tina Van Meter
Dept. of Neuroscience
The Ohio State University
Columbus, Ohio







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