Re: Fixatives for Avian Tissue

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From:Jochen Schuck <>
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Hi Gwin,
why donīt you use 4% Paraformaldehyde in HMSS (Hanks Modified Salt Solution,
which is a Hanks Buffer including magnesium and calcium) or PBS. I used this
even for electron microscopical immunostainings and it worked nicely giving
good ultrastructural preservation and I routinely use it for the paraffin
and cryostuff. By the way I worked on chicken retina and optic nerve....
Good luck and happy staining!

Jochen Schuck

> Von: Gwin Kerce <>
> Antworten an:
> Datum: Tue, 28 Mar 2000 09:22:08 -0500
> An:
> Betreff: Fixatives for Avian Tissue
> Our lab is working on a project that involves locating calcium deposits
> in avian testis.  We have tried to use Bouin's Solution, unfortunately,
> it dissolves the deposits of interest.  We also tried 10% Buffered
> Foramlin, however, the ultrastructure was not preserved.  Does anyone
> have any suggestions for a fixative?  We need to fix both the testicular
> and epididmyal tissue.

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