Re: FW: Cathepsin B

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From:"Mark V. Corl" <>
To:Louise Taylor <>, histonet <>
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Hi Louise-
Mark here from Lab Vision - NeoMarkers...

I went to, and typed in cathepsin B and came up with
the following suppliers:

Enzyme Systems
US Biological

Unfortunately, none of the suppliers list info for IHC.

I also went to and searched MEDLINE for "cathepsin B
immunohistochemistry" and found the following abstract.  Perhaps if you get the
paper, or contact the authors you can find out what antibody they used.

Immunohistochemical analysis of procathepsin L and cathepsin B in cutaneous
Kaposi's sarcoma.
Int J Dermatol 1997 Feb;36(2):100-3   (ISSN: 0011-9059)
Thewes M; Engst R; Jurgens M; Borelli S [Find other articles with these
Department of Dermatology, Technical University of Munich, Germany.

BACKGROUND: The expression of the proteinases cathepsins L and B are induced in
tumors by malignant
transformation, growth factors, and tumor promoters suggesting they play an
important role in tumor invasion
and metastasis. METHODS: By immunohistochemistry, procathepsin L and cathepsin
B were studied in
patients with Kaposi's sarcoma (KS). Formalin-fixed and paraffin-embedded
tissues from 29 cases of KS
(AIDS-associated KS, n = 24; non-AIDS-associated KS, n = 5) were immunolabeled
with the polyclonal
antibody directed against procathepsin L and the antisera directed against
cathepsin B. RESULTS: Normal
epidermis, eccrine sweat glands, and hair follicle expressed both cathepsins.
We also found a positive
staining for procathepsin L in normal blood vessels. In both "angiomatous" and
"fibroblastic" lesions of KS no
expression of these enzymes was observed. CONCLUSIONS: These findings support
the hypothesis of a
benign autochthonous origin of the lesions, such as a hyperplasia.

Good luck,
Mark Corl
Technical Support
Lab Vision - NeoMarkers

Louise Taylor wrote:

> -
> Hi histonetters,
> (I'm resending this message in the hope that someone out there will be able
> to help. I find it hard to belive that no one has used this antibody.)
> Question:
>  Has anyone used Cathepsin B (this IS B and not D) on FFPE human tissue?
> I am particularly interested in the staining pattern, as the spec sheet
> provided does not give an indication as to whether the staining should be
> membranous or nuclear.  The vendor's technical services are not able to
> assist me either, so its up to you guys!
> Thanks in advance
> Louise Taylor
> Research Laboratory
> Department of Anatomical Pathology
> South African Institute for Medical Research
> Johannesburg
> South Africa

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