Re: Antibody precipitate

<< Previous Message | Next Message >> (Peter van de Plas)
Content-Type:text/plain; charset="us-ascii"

Liz Sabin wrote:
>One of the antibodies I'm using appears to have a problem with Ig
>precipitate in the solution.  This appears on the slides as clumps or
>other unexpected deposits of chromagen (in this case, Vector
>VIP).  An obvious solution would be to centrifuge the antibody, so
>can someone suggest duration and rpm?  I'm a bit paranoid about
>spinning down my antibody along with the precipitate!
Dear Liz,
You will certainly loose part of your antibody during centrifugation. Other
options are addition of NaOH (pH between 8 and 9) and/or addition of NaCl

Peter van de Plas
Costerweg 5
6702 AA Wageningen
The Netherlands
phone: (31)-317-497676
fax: (31)-317-415955

<< Previous Message | Next Message >>