Liz Sabin wrote:
>One of the antibodies I'm using appears to have a problem with Ig
>precipitate in the solution.  This appears on the slides as clumps or
>other unexpected deposits of chromagen (in this case, Vector
>VIP).  An obvious solution would be to centrifuge the antibody, so
>can someone suggest duration and rpm?  I'm a bit paranoid about
>spinning down my antibody along with the precipitate!
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Dear Liz,
You will certainly loose part of your antibody during centrifugation. Other
options are addition of NaOH (pH between 8 and 9) and/or addition of NaCl
(0.5-1M).
Peter

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Peter van de Plas
AURION
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