Re: Antibody pre-absorbtion and Methyl Green
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From: | Jochen Schuck <jschuck@otogene.de> |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="ISO-8859-1" |
Wait a minute, wait a minute, my precious liver has other things to do. I
read that methyl greens are pre-cleared in chloroform. I did it once and it
was a glorious mess, but it was clean afterwards.
Dear Dana, can you tell me what light green is. I mostly now the german
names and light green doesn´t ring a bell. What does it stain? Where do I
get it from? How do I use it? I´m still looking for a non-nuclear
counter-stain...
Thanks
Jochen Schuck
> Von: DDittus787@aol.com
> Antworten an: histonet@pathology.swmed.edu
> Datum: Wed, 29 Mar 2000 18:07:51 -0500 (EST)
> An: esabin@srv4.med.ed.ac.uk, histonet@pathology.swmed.edu
> Betreff: Re: Antibody pre-absorbtion and Methyl Green
>
> Most methly greens need to be pre-cleared in cold acetone and cold benzene,
> woops there goes your liver, did you try working light green as a substitute?
> Dana
>
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