For my "thick" sections in EM, (Spurr's medium), I prefer a glass rod with a
ball melted on the end.  Similar to Gayle's wooden stick method in that you
kinda roll the ball in a drop of water to remove the section.
The ball is added by flaming the glass rod, or by ordering them pre made
from any of the EM houses.
Wanda Shotsberger
(HT ASCP)

-----Original Message-----
From: Gary Radice [mailto:gradice@richmond.edu]
Sent: Monday, March 27, 2000 11:02 AM
To: HistoNet Server
Subject: help with transfering plastic sections


We are cutting glycolmethacrylate sections, around 1 micrometer thick, with
glass knives and are having a difficult time transfering sections to
microscope slides without the sections folding. How do you do it/ Does
anyone have a favorite technique they would care to share?

Gary P. Radice			gradice@richmond.edu
Associate Professor of Biology		804 289 8107 (voice)
University of Richmond		804 289 8233 (FAX)
Richmond VA 23173		http://www.science.richmond.edu/~radice