rinse cells with a tris-saline pH 9 buffer containing MgCl2 (5mM). Add
BCIP/NBT (or Fast Red chromogen) to the cells and incubate till adequate
color development is seen (follow under the microscope), wash in PBS and
mount with whatever preferred method you have after counterstaining.
Abizar
www.nnogenex.com


-----Original Message-----
From: Ford, Judi {Path~Palo Alto} [mailto:JUDI.FORD@ROCHE.COM]
Sent: Monday, March 20, 2000 11:08 AM
To: Histonet (E-mail)
Subject: alk phos stain in culture plates


Hi everyone!

I have a question that I'm hoping someone can help me with.  One of our
researchers wants to know of an alkaline phosphastase stain that can be done
in rat bone marrow cultures. Any ideas?

Thanks in advance for your help.

Judi Ford
judi.ford@roche.com