Jay,

Sounds like a good candidate for tyramide signal amplification (NEN Life
Sciences).


Brett M. Connolly, Ph.D.
Merck Research Laboratories
Department of Pharmacology
WP26A-3000
PO Box 4
West Point, PA 19486
Ph. 215-652-2501
FAX 215-652-2075
e-mail: brett_connolly@merck.com

> ----------
> From: 	Jay Turner[SMTP:turnerjayd@hotmail.com]
> Sent: 	Friday, March 24, 2000 5:45 PM
> To: 	histonet@pathology.swmed.edu
> Subject: 	Signal Amplification
> 
> I am staining a fractured rat femur with an immunohistochemical stain to 
> IGF-1.  The stain is a standard biotin-avidin peroxidase with DAB.   The 
> samples were decalcified in Cal-Ex (Fisher), it was a little harsh.  There
> 
> is stain in the section but it is very faint.  I don't want to re-do these
> 
> animals and re-decalcify.  I have tried to use a metal enhanced DAB
> (cobalt) 
>   but the color is too close to the hematoxylin counterstain.  Is there
> any 
> other way to enhance DAB and the staining?  Any suggestions?  Is 
> fluorescence the way to go?
> 
> Thanks!
> 
> ______________________________________________________
> Get Your Private, Free Email at http://www.hotmail.com
> 
>