Neal,

I have tried and had no luck with the biotinylated GAL13 antibody (bought it
from Sigma) on paraformaldehyde fixed paraffin sections, but have yet to
exhaust all protocol possibilities.  So far nil specific binding.  But
please note that I'm no IHC expert.

If/when you do find a good antibody and protocol, please let me know.
	
Phil

> -----Original Message-----
> From: Neal Beeman [mailto:neal.beeman@UCHSC.edu] 
> Sent: Tuesday, March 14, 2000 5:46 PM
> To: Histonet@pathology.swmed.edu
> Subject: LacZ Ab paraffin
> 
> 
> Does anybody know of an antibody against E coli beta galactosidase (Lac Z)
> that works on paraffin sections?  I would especially like an antibody not
> raised in rabbit although at this point I am interested in anything.
> 
> Neal
> 
> 
>