RE: Immuno on Plastic Sections

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From:Pam Marcum <>
To:Donna Kusewitt <>,
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The question is which of the plastics have used or are you looking for a
plastic?  GMA or JB-4 can not be removed from the tissue and will prevent
most if not all IHC staining.  MMA, methylmethacrylate is the best for IHC.
Dr Neil Hand has several papers for the techniques and they are very good.
If I can help call me at 800-523-2575 Ext 134.  Pam Marcum

-----Original Message-----
From: Donna Kusewitt []
Sent: Monday, March 20, 2000 1:20 PM
Subject: Immuno on Plastic Sections

Does anyone have any experience doing immunohistochemistry on plastic
sections - especially those embedded at low temperature?


Donna F. Kusewitt, DVM, PhD
Research Associate Professor
Department of Cell Biology and Physiology
Room 149 BMSB
915 Camino de Salud, NE
School of Medicine
University of New Mexico
Albuquerque, NM  87131
Telephone: 505-272-1302
FAX: 505-272-9105

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