Ann,
I just got this antibody working in my lab.   I use the EDTA at 8.0 in a
steamer for 45 minutes, cool to room temperature.   I use a DAKO
autostainer.   The dilution I used was 1:25 and incubated for 30 minutes.
I have the phone number of the guy who originally worked with this antibody.
If you want to call him let me know.   Hope this helps.    (by the way, he
uses a microwave for his AR)
Hazel

> -----Original Message-----
> From:	ANN MARUSKA [SMTP:amarusk1@FAIRVIEW.ORG]
> Sent:	Thursday, March 23, 2000 04:51 PM
> To:	histonet@pathology.swmed.EDU
> Subject:	Alk Protein
> 
> Hi Histonetters!
> Anyone out there doing Alk Protein (for anaplstic large cell lymphomas) on
> the NexES/ES?  I have tried using Dako's antibody by steaming & decloaking
> in both EDTA 8.0 and Tris-urea 9.5 and with both the basic and enhanced
> DAB kit - no luck!  I am using a piece of tissue that was confirmed to be
> positive by an outside lab, so I don't think that is an issue.  Any
> suggestions?
> TIA
> Ann Maruska
> Fairview-University Med Ctr
> Mpls. MN
> amarusk1@fairview.org 
> 
> 
> 
>