I have cut thick paraffin sections of skin by cutting very slowly while
holding down the leading edge of the section with a cake-testing stick
(thinner than an "applicator stick").
						Allen A. Smith
						Barry University
						Miami Shores, FL

		-----Original Message-----
		From:	Maureen Decorah [mailto:decorah@rarc.wisc.edu]
		Sent:	Thursday, March 23, 2000 1:21 PM
		To:	HISTONET
		Subject:	40 micron sections

		Received this question but I don't have any experience
cutting this thick.
		Does anyone have suggestions for him?



		 I am trying to cut 40 micron sections of brain tissue
(mouse)
		>embedded in paraffin.  The sections don't come off the
knife well and if I
		>do get them they crack later.  I seem to be able to get 5
micron sections
		>from the same block ok.  Is it possible to get 40 micron
sections and if so
		>are there any "tricks" to doing it?  Thanks again for your
help.
		>



	
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
			Maureen Decorah, Research Animal Resources Center
			385A Enzyme Institute, 1710 University Avenue
			University of Wisconsin - Madison, Madison, WI
53705-4098
			(608) 262-0933  Fax(608) 265-2698  E-mail:
decorah@rarc.wisc.edu
	
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