HMB45 vs Schmorls

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From:amos brooks <>
To:histonet <>
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<!doctype html public "-//w3c//dtd html 4.0 transitional//en"> <html> Hi, <br>    Yesterday, someone (I forgot who, I went delete happy) asked the difference between the Schmorls histochemical (HC) stain and the immunohistochemical (IHC) stains S100 and HMB45. <br>    The main difference is specificity. The immuno methods are very specific, HMB45 stains only melanosomes, and S100 stains only neurologically derived cells. Schmorls will stain reducing substances. This means melanin, argentaffin and any substance with a lower pH than the staining solution. <br>    Sheehan notes in <u>Theory and Practice of Histotechnology</u> that, "A positive reaction is presumptive evidence of the presence of melanin, though other reducing substances react, including ascorbic oxalic and uric acids, phenols, indoles, aryl amines, thiols and others." (pg 223) <br>    I do think that some of these results could be done without the benefit of the IHC techniques, but there are occasions where the IHC is needed and preferred over the HC method. <br>Amos Brooks</html>
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