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Dear Netters,
<p>We are having differentiating problems with our Gaffney's One-Hour Giemsa
stain. We purchased our Giemsa stock solution from Newcomer supply.
We are following the AFIP protocol which is very simple. The AFIP
calls for 10 ml of Giemsa stock to every 50 ml of Acetic water solution.
Acetic water solution is 1 drop Glacial Acetic Acid to 1000 ml of distilled
water. We deparaffinize and hydrate to distilled water then, stain
in the Giemsa working solution for one hour. The stain is not differentiating
between cytoplasm (pink), nuclei (blue) and erythrocytes (red). Do
you think perhaps the Acetic water solution is too weak? Does anyone
else use the AFIP protocol? If so, does it work for you? Any
input on this matter would be appreciated.
<p>Sincerely,
<p>Beth Poole
<br>Histo-Scientific Research Labs.
<br>107 Killmon Road
<br>Basye, VA 22810
<br>(540)856-2222
<br>histosci@shentel.net</html>