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From:Louise Taylor <louiset@mail.saimr.wits.ac.za>
To:histonet <histonet@pathology.swmed.edu>
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Dear histonetters

I have some questions regarding citrate buffer that have been keeping me
awake o'nights.....:)

Can citrate buffer pH 6.0 be made up in advance? We usually prepare it daily
from stock solutions of citric acid and sodium citrate in quantities
sufficient only for the slides being microwaved.

Can we make up - say 5liters- and keep it in the fridge? How long could a
bulk solution be kept this way?
Can solution that has been in the microwave be used again - or is there a
change in pH or molarity? How often could one "recycle" the buffer?

Thanks in advance for any input

Best regards
Louise taylor

Research Laboratory
Department of Anatomical Pathology
South African Institute for Medical Research
South Africa

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