We cut coronal hemispheres of human brain on a motorised microtome. It's an
old LKB, but it works fantastically well.  All brains are embedded in
paraffin, and cut at between 7-10um.

The new motorised poly cuts can apparently do the same.

Cheers

Jim Manavis
Department of Neuropathology
Institute of Medical & Veterinary Science
Adelaide, SA, 5000
Australia
Phone: 61-08-8222-3588
FAX: 61-08-8222 3204
e.mail: jim.manavis@imvs.sa.gov.au
Disclaimer: Not this little black duck!

-----Original Message-----
From: L. Gibbs <lgibbs@u.washington.edu>
To: A. Erickson <andrae@u.washington.edu>
Cc: HistoNet Server <HistoNet@pathology.swmed.edu>
Date: Friday, 17 March 2000 9:44
Subject: Re: Automatic (robotic?) microtomes


>Hi Andra-
>
>I once did large paraffin blocks of brain on the sledge with a
>250mm knife. It worked but there may be a better way. Yes, Donna
>would know. You might try contacting Neuropathology.
>
>Good luck!
>
>
>Lorraine Gibbs
>
>On Thu, 16 Mar 2000, A. Erickson wrote:
>
>> Ooonce again, Im in need of info that was probably discussed on Histonet.
>> Any recomendations on automatic microtomes from those who use
>> them...including pros & cons, vendors and phone numbers.  An investigator
>> Im working with now wants to do large paraffin blocks of primate brain.
>> Anyk suggestions would be appreciated!  Donna Simmons, are you out there?
>> Andra Erickson, University of Washington RPRC, Seattle, Wa.
>>
>>
>>
>
>
>