Alcian Blue question

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From:Carol Bobrowitz <Carol_Bobrowitz.PATHOLOGY@qmail.path.mcw.edu>
To:HistoNet <histonet@pathology.swmed.edu>
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                      Alcian Blue question
I am doing an Alcian Blue - PAS - Hematoxylin Stain.  I have not had alot of
experience with Alcian Blue.  My questions are:  
1) Does differenciation in Acid Alcohol remove the Alcian Blue Stain?
2) Instead of using Harris Hematoxylin should I use Mayers?
Any tips will be appreciated and thanks to everyone in advance.
Carol Ann Bobrowitz
Medical College of Wisconsin
carol_bobrowitz@path.mcw.edu





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