re: ways to recover antigens...

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From:Shelley Sheridan <>
To:HistoNet Server <>
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We do immunohistochemsitry routinely and with some
antibodies we need to do antigen retrieval.  There are
three treatments that we use.

1.  Treat in 88% Formic Acid

2.  Microwave

3.  Boil in citrate buffer

Some work better than others depending on the
antibody.  If you want more specific information let
me know.  Good luck.

Shelley K. Sheridan
Research Specialist
Center for Neurodegenerative Disease Research
University of Pennsylvania Medical School
Philadelphia, PA 19104
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