re: ways to recover antigens...

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From:Shelley Sheridan <sksherid@yahoo.com>
To:HistoNet Server <histonet@pathology.swmed.edu>
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We do immunohistochemsitry routinely and with some
antibodies we need to do antigen retrieval.  There are
three treatments that we use.

1.  Treat in 88% Formic Acid

2.  Microwave

3.  Boil in citrate buffer

Some work better than others depending on the
antibody.  If you want more specific information let
me know.  Good luck.



=====
Shelley K. Sheridan
Research Specialist
Center for Neurodegenerative Disease Research
University of Pennsylvania Medical School
Philadelphia, PA 19104
Phone:(215)614-0051
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