re: ways to recover antigens...
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From: | Shelley Sheridan <sksherid@yahoo.com> |
To: | HistoNet Server <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
We do immunohistochemsitry routinely and with some
antibodies we need to do antigen retrieval. There are
three treatments that we use.
1. Treat in 88% Formic Acid
2. Microwave
3. Boil in citrate buffer
Some work better than others depending on the
antibody. If you want more specific information let
me know. Good luck.
=====
Shelley K. Sheridan
Research Specialist
Center for Neurodegenerative Disease Research
University of Pennsylvania Medical School
Philadelphia, PA 19104
Phone:(215)614-0051
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