ihc for beta-gal

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Can you recommend a good method for beta-gal IHC?  We have been using the
Sigma GAL-13 antibody which is biotin labeled on tissue briefly fixed in 2%
paraformaldehyde without success.  The tissue has been enzyme cytochemically
reacted with a substrate for beta-gal before fixing and processing into
paraffin.  Do you suppose this enzyme cytochemical staining is preventing
the IHC labeling of the antigen?  Please respond directly to
Philip.Hardy@uchsc.edu on this one.
Patsy Ruegg

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