hair cell IHC

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Fellow netters:

One of the post docs here is doing IHC on frozen sections of frog sacculi.  The 
staining works well, but the morphology of the hair cells is horrible.  Maybe someone 
has a suggestion on how to preserve the integrity of these cells though the 
cryosectioning/staining process.  We embed the sacculus in agar, sink in 30% sucrose, 
and freeze slowly over liquid nitrogen.  For most small specimens we work with, this 
procedure works well, but the sacculus seems to be more picky-you-nish.  So if anyone 
out there works with this organ, and has ideas on how to improve our morphology, I'd 
love to hear from you.


Karen in Oregon

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