I would appreciate hearing from anyone who has this marker working reliably to learn
1. who's antibody you use
2. your antigen recovery method
3.  any caveats or tricks you might offer to provide better reliability
4. what you are using for a control

we have had some cases stain very well, others that the pathologist insists should be positive that weren't.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue
Suite 309
Charleston, SC  29425
ph:  (843) 792-6353
fax: (843) 792-8974
email: Dellav@musc.edu