Re: ways to recover antigens...

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From:amos brooks <atbrooks@snet.net>
To:Shelley Sheridan <sksherid@yahoo.com>
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Hi,
    Enzymatic predigestion can be done at room temperature. Try
Proteinase K or Pepsin. I know DAKO has these in ready to use form and I
am sure many others do too.
Amos Brooks

Shelley Sheridan wrote:

> Maria
>
> The only room temperature antigen retreival method
> that we use is 1 minute in 88% Formic Acid.  The other
> two methods that we use require heating.
>
> =====
> Shelley K. Sheridan
> Research Specialist
> Center for Neurodegenerative Disease Research
> University of Pennsylvania Medical School
> Philadelphia, PA 19104
> Phone:(215)614-0051
> __________________________________________________
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