Re: transfer question
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From: | amos brooks <atbrooks@snet.net> |
To: | Lynn Gardner <lynn-gardner@uiowa.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Hi Lynn,
We do a lift off procedure in which a stained slide can have the coverslip
removed and a gel placed on the sections. The slide is then left in a 60 degree
oven overnight then placed in a coplin jar of distilled water in a float bath
for an hour. The sections can then be floated off the slide on the float bath,
split up on two separate slides and restained. Unfortunately I forgot the name
of the darned gel used for this. I'll get it to you Monday afternoon along with
a more detailed description of the procedure.
Amos Brooks
Lynn Gardner wrote:
> Hey histonetters! Have another question for the group. Is there a way to
> remove a 4 micron formalin fixed paraffin embedded section from a plus
> slide after deparaffinization and keeping the section intact in order to be
> able to stain it as a free floating section? Sure look forward to your
> answers! Thanks all for your help.
>
> Sincerely,
> Lynn Gardner
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