Re: mouse organ fixation

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From:Jamie Erickson <>,

We do lots of mouse work, I fix in 10%NBF for 24 hours the samples are
cut in to get maximal fixation. 

Jamie Erickson
Associate Scientist
Genetics Institute
1 Burtt Rd.
Andover, MA   01810
work : (978) 247-1348
FAX  : (978) 247-1333

>>> "Yandl, Emily" <> 03/06 4:51 PM >>>
Hi all!

We have a researcher here who is submitting normal whole mouse
(liver, heart, lung, kidney) for paraffin processing for use as IHC
controls.  Currently, she is fixing these organs in 10%NBF for 4
hours to
retain as much immunogenicity as possible, however, I am worried that
organs are not completely fixing and are drying out in the alcohols. 

What fixation procedures do those of you who work with mouse tissue
How long in fix when doing IHC?  Are the organs grossed open prior
fixation or left whole?

Thanks for any help!

Emily Yandl
Pre-Clinical Biology
Genzyme Corporation
PO Box 9322
Framingham, MA  01701
(508) 872-8400 (x23623) 

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