---------- Forwarded message ----------
Karen,

Did he decalcify the tissue?  Most saccular macculi have calcified
otoconia on their surface (in the otoconial membrane).  I have done some
IHC on frozen sections of chinchilla inner ear, but I prefer the
preservation of structure that you get with paraffin.  Can he embed in
paraffin or is the antibody sensitive to it?  

Karen Pawlowski
UT Southwestern Med. Ctr.
Dallas, TX

Karen S Pawlowski wrote:
> 
> ---------- Forwarded message ----------
> Date: Wed, 01 Mar 2000 12:17:09 +0000
> From: Karen Larison <larisonk@uoneuro.uoregon.edu>
> To: HistoNet@Pathology.swmed.edu
> Subject: hair cell IHC
> 
> Fellow netters:
> 
> One of the post docs here is doing IHC on frozen sections of frog sacculi.  The
> staining works well, but the morphology of the hair cells is horrible.  Maybe someone
> has a suggestion on how to preserve the integrity of these cells though the
> cryosectioning/staining process.  We embed the sacculus in agar, sink in 30% sucrose,
> and freeze slowly over liquid nitrogen.  For most small specimens we work with, this
> procedure works well, but the sacculus seems to be more picky-you-nish.  So if anyone
> out there works with this organ, and has ideas on how to improve our morphology, I'd
> love to hear from you.
> 
> Thanks,
> 
> Karen in Oregon