Re: bone marrow smears

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From:amos brooks <>
To:lizellis <>
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    We always did Wright - Giemsa methods following a fixation in methanol.
It has been noted that some hematologists were inept at making the smears,
often missing the spicules entirely while others were consistently good at
it. This may be part of the problem. Make sure the smears are done right and
good results will probably follow.
Amos Brooks

lizellis wrote:

> Has anyone out there found a good manual staining method for bone marrow
> smears which accompany a bone marrow biopsy?
> We have tried Diff-Quik, a long Giemsa procedure and a Wright-Giemsa one
> step method, all with mixed reviews.
>  The Pathologist can only seem to read the smear 50% of the time. We have
> even tried  the Hem-Tek automated stainer in our Hematology Dept., again
> with mixed reviews. Any insight would be greatly appreciated.
> Liz Ellis,
> Histology Manager
> Quest Diagnostics, Horsham ,PA

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