Re: anti-rat antibodies

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From:Barbara Wright <>
To:Louise Taylor <>
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We use DAKO Catalogue # Z0628 polyclonal S100 on rat and mouse tissue at a
concentration of 1:4000. for 30 minutes.   Pretreatment in Citra at 99C for 10
minutes is required.  If you would like a copy of the protocol, I would be glad
to email it to you.


Louise Taylor wrote:

> Hi histonetters,
> I need some help please. I have been asked to do S100 on rat tissue. I have
> no idea where to start looking fo animal primaries, as we generally do
> antihuman studies. I would also like some information on what secondary o r
> amplification kits would be appropriate. Many thanks
> Louise taylor
> Research Laboratory
> Department of Anatomical Pathology
> South African Institute for Medical Research
> Johannesburg
> South Africa

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