Date sent:      	Wed, 01 Mar 2000 12:46:50 +0200
From:           	Louise Taylor <louiset@mail.saimr.wits.ac.za>
Subject:        	anti-rat antibodies
To:             	histonet <histonet@pathology.swmed.edu>

> 
> Hi histonetters,
> 
> I need some help please. I have been asked to do S100 on rat tissue. I have
> no idea where to start looking fo animal primaries, as we generally do
> antihuman studies. I would also like some information on what secondary o r
> amplification kits would be appropriate. Many thanks

Louise,
I have used S100 (cow, from DAKO) on mouse tissue and achieved 
similar results to that of human (same protocol,no 
amplification).This protein is common in a number of species and 
when used as a rabbit polyclonal (swine anti rabbit bridge) there 
should be no non specific binding to your rat tissue.

Terry.


Terry Hacker,
Medical Research Council,
Harwell,
Didcot,
Oxfordshire, OX11 ORD
01235 834393 x360