Re: anti-rat antibodies

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From:"" <>
To:Louise Taylor <>

Date sent:      	Wed, 01 Mar 2000 12:46:50 +0200
From:           	Louise Taylor <>
Subject:        	anti-rat antibodies
To:             	histonet <>

> Hi histonetters,
> I need some help please. I have been asked to do S100 on rat tissue. I have
> no idea where to start looking fo animal primaries, as we generally do
> antihuman studies. I would also like some information on what secondary o r
> amplification kits would be appropriate. Many thanks

I have used S100 (cow, from DAKO) on mouse tissue and achieved 
similar results to that of human (same protocol,no 
amplification).This protein is common in a number of species and 
when used as a rabbit polyclonal (swine anti rabbit bridge) there 
should be no non specific binding to your rat tissue.


Terry Hacker,
Medical Research Council,
Oxfordshire, OX11 ORD
01235 834393 x360

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