Re: Lac-Z/B-gal - reply

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From:Robert Schoonhoven <>
To:Luis Chiriboga <>
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I use the anti B-Galactosidase from Boehringer-Mannheim, cat# 1083 104.

dilution 1:100, incubate at room temp. for 10 minutes, demonstrate with
Dako Envision kit (secondary antibody, 10 min. RT)

We have used this antibody for a few years now and it works very nicely
and is consistant.  Our tissues are fixed in 10% NBF and we do not use
any pretreatment (HEIR or enzymes).

Luis Chiriboga wrote:
> Hi netters;
> does anyone know of an antibody/source against beta-gal thant can be
> used in tissues?
> Thanks in advance

best regards,
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
Chapel Hill, NC 27599
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123 

Don't go around saying the world owes you a living; the world owes you
nothing; it was here first. 
Mark Twain [Samuel Langhornne Clemens] (1835-1910)

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