Re: IHC question

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Dear Ann,

I often remove film from H&E slides to do IHC. I soak the slide in acetone 
for 5 minutes to remove the coverslip film.  Then 3 changes of absolute 
alcohol for 30 seconds each to remove the remainder of the adhesive, followed 
by 3 changes of xylene, 1 minute each.  I then rehydrate thru absolute 
alcohol and 95% alcohol to water, then place in buffer.  No need to 
decolorize.  I've had very good luck doing IHC on these slides. I often get 
H&E slides for CKHMW, and occasionally for other antibodies.  The Dr.s have 
been very pleased.

Ellen Yee
Central Histology Facility
Sacramento, CA

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