Re: Fixation time for mouse skin

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I am asking this question for a graduate student here in our lab...Some mouse
skin was recently processed and when trying to cut the sections it was found
that the tissue was too brittle.  The skins were fixed in 10% nbf for 16 hours
and then dehydrated in a series of ethanol dilutions(70%, 85%, 90% and 100%) for
20 minutes each.  Skin was then cleared in xylene for 20minutes, dipped in
parrafin and then embedded.  We think that perhaps the skin was fixed for too
long a period.  Does anyone have any suggestions regading times for the
fixation, clearing or parrafin infiltraion?
I realize that this question has probably been asked many times before, but I am
relatively new to the field of histology and am still learning where to find
good accurate information.
Thanking you in advance,
I remain sincerely yours,
Christine Klein
MD Anderson Cancer Center Houston Texas

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