Re: Filipin Stain For Fat? (cholesterol)
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Keith Rogers <krogers@ncifcrf.gov> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Fri, 10 Mar 2000, Keith Rogers wrote:
> Our pathologist dug this one up somewhere but has no reference. He assures
> me it is Filipin and not flipin!
> Any suggestions would be greatly appreciated.
Filipin is an organic compound (an antibiotic, I think) that
binds specifically to cholesterol. Bound filipin causes a
characteristic structural change or "decoration" that can be
detected by electron microscopy, especially in freeze-fracture
preparations of cell mambranes. Filipin has also been used to
block affinity-based staining of cholesterol with other reagents,
at the EM level.
These statements are based on notes taken from two papers (1994 and
1997) in the J. Histochem. Cytochem. I recommend that you do a
PubMed search to get a more balanced selection of publications
on the subject - http://www.ncbi.nlm.nih.gov/pubmed
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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