Re: Decal Solutions

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From:"Bill Sinai (Anatomical Pathology)" <Bills@icpmr.wsahs.nsw.gov.au>
To:histonet@pathology.swmed.edu
Reply-To:
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Date:          Tue, 29 Feb 2000 12:45:16 -0500
From:          Vinnie Della Speranza <dellav@musc.edu>
Subject:       Re: Decal Solutions
To:            CHRISTA.HLADIK@email.swmed.edu
Cc:            histonet@pathology.swmed.edu

I agree with Vinnie, but we have been using a 5% solution of Nitric 
Acid which appears to allow most of the antigenic sites still 
available.

*********************************************************************
 A word of advice. if you are planning to do immuno in 
these specimens, I would recommend nothing stronger than 5% Formic 
acid. Our bone marrow cores take about 1  - 2 hrs to decal in this 
solution. Vinnie

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue
Suite 309
Charleston, SC  29425
ph:  (843) 792-6353
fax: (843) 792-8974
email: Dellav@musc.edu

>>> Christa Hladik <CHRISTA.HLADIK@email.swmed.edu> 02/28/00 10:45AM >>>
Hello,
Our lab has been asked to do a project which includes decal of bone marrow biopsies.  
It has been years since I have worked in routine, I really could use some updating on decal solutions that are being used today and approximate times in solutions.  

Thanks in advance for your help!

Christa Hladik, HT(ASCP) QIHC
Technical Director, Pathology Immunohistochemistry Laboratory
University of Texas Southwestern Medical Center
6000 Harry Hines Blvd.
Lab NB7.214
Dallas, Texas  75235-9072
(214) 648-1177; Fax (214) 648-1179
e-mail:  christa.hladik@email.swmed.edu 




Bill Sinai
Department Manager
Tissue Pathology
ICPMR Westmead Hospital 
WESTMEAD NSW AUSTRALIA
Phone 61+2+9845 7774  Fax 61+2+9687 2330



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