Re: Cryosectioning

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From:Mary Latimer <>
To:"tony.whyte" <>
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Dear Tony  I have read a few replys to your problem I would just like to
ask how long have they been frozen ...May a degree of freeze drying be
going on? changing the angle of the knife may well help too? I take it
that the oct surround is cutting well?  Good Luck....Mary

On Wed, 8 Mar 2000, tony.whyte wrote:

> Hi all,
> i'm having trouble sectioning whole frozen mouse embryos.  They were snap
> frozen in isopentane in liq N2 and mounted in OCT on cork.  The trouble is
> either that the section is compressing or else fragmenting.  Using a new knife
> hasn't helped the problem.  Any ideas?
> Tony W.
> Cambridge

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