tony.whyte wrote:
> 
> Hi all,
> 
> i'm having trouble sectioning whole frozen mouse embryos.  They were snap
> frozen in isopentane in liq N2 and mounted in OCT on cork.  The trouble is
> either that the section is compressing or else fragmenting.  Using a new knife
> hasn't helped the problem.  Any ideas?
> 
> Tony W.
> Cambridge


Hi Tony
I have found that I get the best sections when my cyrostat is at -30 to
-35. I get all stages of embros right on up to newborns, and this works
for me. Good Luck
Robbin