Re: Cryosectioning

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From:"Robbin Newlin Manager, Histology Core Facility" <>
To:"tony.whyte" <>
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tony.whyte wrote:
> Hi all,
> i'm having trouble sectioning whole frozen mouse embryos.  They were snap
> frozen in isopentane in liq N2 and mounted in OCT on cork.  The trouble is
> either that the section is compressing or else fragmenting.  Using a new knife
> hasn't helped the problem.  Any ideas?
> Tony W.
> Cambridge

Hi Tony
I have found that I get the best sections when my cyrostat is at -30 to
-35. I get all stages of embros right on up to newborns, and this works
for me. Good Luck

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