Re: Cryosectioning

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From:Joyce Kotzuk <>,
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Tony, When I used to section fresh frozen mouse or rat brains, I would place my gloved thumb on the face of the block for a few seconds to warm it ever so slightly just before taking a section. This would prevent breakage of the section, or "chatter" as we called it, and produce a beautiful, complete and untorn section. Maybe this little tip will help you.
Joyce Kotzuk, UNM pathology dept.

>>> "tony.whyte" <> 03/08/00 08:21AM >>>
Hi all,

i'm having trouble sectioning whole frozen mouse embryos.  They were snap
frozen in isopentane in liq N2 and mounted in OCT on cork.  The trouble is
either that the section is compressing or else fragmenting.  Using a new knife
hasn't helped the problem.  Any ideas?

Tony W.

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