Re: CD-117

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From:Donald Andersen <>,
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Mr. Nocito;
Dako's polyclonal Ab (A4502) works well for us in formalin fixed, paraffin embedded tissue. We pretreat by heating sections in tris buffer at pH-10 and incubate overnight at 1:2,000. (1:100 to 1:200 should work for 1 hour incubation.) The antibody also works well on lightly (surface) demineralized blocks.

Donald Andersen
Histochemistry Laboratory
Oregon Health Sciences University

>>> "Nocito, Joseph" <> - 3/13/00 5:14 AM >>>
Hello netters,
on a recent CAP survey for IHC, CD-117 was one of the antibodies requested,
looking through the catalogs, I've spotted several clones and species.
Because of budget restraints, I can't perform an adequate screening of three
or four different CD-117 antibodies.  Does anyone use CD-117 on
formalin-fixed, paraffin embedded human tissue? Is it worth trying? I've
seen monoclonal and polyclonal antibodies. Which one works better.  I thank
you in advance for your help.  Have a happy and safe day!! 

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas 

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