Re: Amyloid Staining

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From:Susan Meloan <smeloan@mail.mcg.edu>
To:histonet@pathology.swmed.edu, dbrocato@uic.edu
Reply-To:
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We use a Potassium Permanganate method here. The reference is Wright JR, Clakins E, ZHumphrey RL: Potassium Permanganate reaction in amyloidosis: a histological method to assist in differentiating forms of this disease.  Lab Invest. 36:274-281, 1977



Susan Meloan
Chief Histotechnologist
Department of Pathology
Medical College of Georgia
Augusta, Ga 30912
(706) 721-3630
e-mail: smeloan@mail.mcg.edu



>>> dbrocato <dbrocato@uic.edu> 02/29 7:14 AM >>>
Hello histonetters!!

I am doing a routine Congo Red stain for Amyloid deposits, but has anyone out 
there ever heard of a Potassium Permanganate 1% dip to bleach and distinguish 
between primary and secondary amyloid??? This may be an old procedure.  Any 
help would be greatly appreciated!!  Please e-mail me directly at 
dbrocato@uic.edu.

Thanks all!

Dave Brocato, BS, HT(ASCP)






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