RE: mouse organ fixation

<< Previous Message | Next Message >>
From:Catherine_Robertson@hc-sc.gc.ca
To:Cynthia Favara <cfavara@niaid.nih.gov>
Reply-To:
Content-Type:text/plain; charset=us-ascii



To expand on the topic of mouse organ fixation, I am involved in setting up a
prion pathogenesis unit. Part of our work will involve a small amount of
histology and Immunohistochemistry on mouse brains and spleens.  I have a few
problems with the availability of space and the limit on the amounts of solvents
that can be kept in the lab.
I have a couple of questions.
1.   Is there a small benchtop processor available that doesn't use as much
solvents as the full size processors?
2.   Can I use a laboratory microwave to process these tissues, and if so, does
anyone have any protocols for this?

Thanks,

Cathy Robertson
Host Genetics and Prion Diseases
Health Canada
Winnipeg MB
Canada





<< Previous Message | Next Message >>