Hello Louise,
InnoGenex carries an anti- S-100 Protein antibody that is validated on rat,
mouse and human tissue(Catalog No. AM-2435-11). We also produce IHC kits for
rodent tissue and carry all of the appropriate pre-adsorbed secondary
antibodies.
http://www.innogenex.com
Best Regards,
Matthew Ogdie
InnoGenex
(925)543-1414


-----Original Message-----
From: t.hacker@har.mrc.ac.uk [mailto:T.Hacker@har.mrc.ac.uk]
Sent: Wednesday, March 01, 2000 8:37 AM
To: Louise Taylor
Cc: 'Histonet'
Subject: Re: anti-rat antibodies


Date sent:      	Wed, 01 Mar 2000 12:46:50 +0200
From:           	Louise Taylor <louiset@mail.saimr.wits.ac.za>
Subject:        	anti-rat antibodies
To:             	histonet <histonet@pathology.swmed.edu>

>
> Hi histonetters,
>
> I need some help please. I have been asked to do S100 on rat tissue. I
have
> no idea where to start looking fo animal primaries, as we generally do
> antihuman studies. I would also like some information on what secondary o
r
> amplification kits would be appropriate. Many thanks

Louise,
I have used S100 (cow, from DAKO) on mouse tissue and achieved
similar results to that of human (same protocol,no
amplification).This protein is common in a number of species and
when used as a rabbit polyclonal (swine anti rabbit bridge) there
should be no non specific binding to your rat tissue.

Terry.


Terry Hacker,
Medical Research Council,
Harwell,
Didcot,
Oxfordshire, OX11 ORD
01235 834393 x360