RE: amyloid staining
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From: | "Richard J. Blais" <blaisrj@hotmail.com> |
To: | Histonet@Pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; format=flowed |
Following the reference I gave your yesterday, here is the procedure in use
in my lab:
Primary amyloid (AL) is not affected by an oxydation by potassium
permanganate, but secondary amyloid (AA) is.
Prior to staining with Congo Red, the slides are oxydised in a solution of
aquous potassium permanganate 0.5%(w/V) mixed in equal part with an aquous
solution of sulphuric acid 0.3%(v/v) for 2.5 minutes. The slides are then
washed thoroughly, and bleached in an aquous solution of oxalic acid
0.5%(w/v).
Primary amyloid will still stain with Congo Red, but secondary won't.
Ref: John D. Bancroft, Harry C. Cook, Manual of histological techniques and
their diagnostical applications.
Hope this will help you.
Richard Blais
blaisrj@hotmail.com
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