Keith,

It depends on the fixative and demineralization used.
Isoform 5 of Acid Phosphatase(TrAP) seems to be more resistant to FFPE than
other Isoforms.
For NBF fixed, +/-  demin. in Formic acid. we find that extending the
incubation in substrate(Barka modification of Burstone Napthol AS-BI method)
from 60 min to overnight, will work well. We also use IHC with the antibody
from Zymed. This works well on NBF +/- demin. and on AZF +/- demin. One can
always resort to IHC using CD68, which demonstrates Osteoclasts beautifully!



Bryan
> ----------
> From: 	Keith Rogers[SMTP:krogers@ncifcrf.gov]
> Sent: 	March 2, 2000 12:33 PM
> To: 	HistoNet@pathology.swmed.edu
> Subject: 	Trap stain for Osteoclasts
> 
> Does anyone have a reliable alternative to the Trap method that does not 
> involve the use of soft paraffin? is there perhaps an antibody technique?
> 
> Thanks in advance,
> 
> Keith Rogers
> Frederick Cancer Research Inst. 
>