RE: IHC question

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To:settembr@UMDNJ.EDU, amarusk1@FAIRVIEW.ORG

I soak the slides in acetone for 2 - 3 mins, lift off the coverslip then let
the sections soak in fresh acetone for 5 - 10 mins to remove any residual
film.  Then proceed as normal.  

Penny Marr
SEALS - St George Hospital
Gray St
Kogarah  NSW  2217

> -----Original Message-----
> From:	Dana Settembre [SMTP:settembr@UMDNJ.EDU]
> Sent:	Tuesday, 14 March 2000 0:11
> Cc:
> Subject:	Re: IHC question
> On Fri, 10 Mar 2000, ANN MARUSKA wrote:
> > Dear Histonetters,
> > For those of you who do IHC stains and get slides that have been
> previously stained e.g. H&E, how do you get the coverslip film off the
> slides?  I know there is a quick method by placing the slides in acetone,
> but does this affect the antigenicity of those slides? 
> > Right now I soak them in Xylene, but it takes days to get the film
> unglued.  Glass coverslips come off rather quickly in xylene, it's just
> the film coverslip that takes forever.
> > Thanks for whatever info you can share.
> > Ann Maruska
> > Fairview-University Med Ctr
> > Mpls.  MN  55454
> > 
> > 
> > 
> > 
> Hello Ann,
> I sometimes run IHC on H&E slides and have had trouble with "film"
> coverslip.  But soaking them in the "original" reagent that came before
> coverslipping is the key.  This is lab we use xylene just before
> coverslipping and in the lab I used to work at, used  Histoclear just
> before coverslipping.  Sometimes I don't know which reagent was used by
> another lab and you have to fiddle with several reagents.  You also must
> go back to try to move the coverslip a bit quite often.  Hope this helps
> Dana Settembre
> Immunohistochemistry Lab
> Pathology Department
> University Hospital
> Newark,  New Jersey
South Eastern Sydney Area Health Service

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